Type III collagen protein, collagen 3 protein, human collagen protein, Alpha 1 type III collagen protein, Alpha1 (III) collagen protein, COL3A1_HUMAN protein, Collagen Type III Alpha 1 Chain protein, Ehlers-Danlos Syndrome Type IV Autosomal Dominant protein, Fetal Collagen protein, Human placenta Collagen Type III Protein protein, Human Type III Collagen Protein protein, scleroprotein collagen protein
Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with Anti-Collagen Type III. Reaction with Anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Form:
Liquid (sterile filtered)
Target:
COL3A1
Application Dilute:
IF: 1:100-1:500, IP: 1:100-1:500, WB: 1:1000
Application Notes:
Biological Origin: Human. Application Notes: Human Collagen Type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure
SDS-PAGE Results of Human Collagen Type III. Lane 1: Human Collagen Type III Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human Collagen Type III Non-Reduced [1.0 µg]. 4-20% Gel Coomassie Stained. Observed: alpha1 bands.
Western Blot of COLLAGEN III. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.
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