200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Form:
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Target:
NT5E
Application Dilute:
ELISA (For coating, order antibody without BSA),Flow Cytometry (2-4ug/million cells in 0.1ml),,Immunofluorescence (2-4ug/ml), Western Blot (2-4ug/ml), ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissu
Application Notes:
Application Notes: Positive Control: A431, U87MG or PC-3 cells. Human tonsil, prostate or lung carcinoma
Analysis of Protein Array containing more than 19000 full-length human proteins using CD73-Monospecific Mouse Monoclonal Antibody (CD73/2646). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2646).
Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2646) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin (red).
SDS-PAGE Analysis Purified CD73 Mouse Monoclonal Antibody (NT5E/2646). Confirmation of Integrity and Purity of Antibody.
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