Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target:
S100A4
Application Dilute:
Flow Cytometry (5ul/million cells), Western Blot (2-4ug/ml), Immunofluorescence (2-4ug/ml at RT),,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT),,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9
Application Notes:
Application Notes: Positive Control: HeLa, A549 or A375 cells. Human placenta tissue
Analysis of Protein Array containing more than 19000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/1481). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow Cytometric Analysis of A549 cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Flow Cytometric Analysis of T98G cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/1481). Confirmation of Integrity and Purity of Antibody.
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