Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target:
CD47
Application Dilute:
Immunofluorescence (2-4ug/ml), Flow Cytometry (5ul per million cells),,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mi
Application Notes:
Application Notes: Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC)
Analysis of Protein Array containing more than 19000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow Cytometric Analysis of PFA-fixed MCF-7 cells using CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.
SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/3019). Confirmation of Integrity and Purity of Antibody.
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