Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target:
PAX5
Application Dilute:
Western Blot (2-4ug/ml), ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 C followed by cooling at RT for 20 mi
Application Notes:
Application Notes: Positive Control: Raji, DAUDI or Ramos cells. Human tonsil, spleen or lymph node
Analysis of Protein Array containing more than 21000 full-length human proteins using PAX5 Mouse Monoclonal Antibody (PAX5/3735) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Formalin-fixed, paraffin-embedded human Tonsil stained with PAX5 Mouse Monoclonal Antibody (PAX5/3735).
Formalin-fixed, paraffin-embedded human Tonsil stained with PAX5 Mouse Monoclonal Antibody (PAX5/3735).
SDS-PAGE Analysis Purified PAX5 Mouse Monoclonal Antibody (PAX5/3735). Confirmation of Purity and Integrity of Antibody.
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