Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target:
TIGIT
Application Dilute:
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 C followed by cooling at RT for 20 minutes),Optimal dilution fo
Application Notes:
Application Notes: Positive Control: Human tonsil or prostate carcinoma
Analysis of Protein Array containing more than 19000 full-length human proteins using TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3106). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDAs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDAs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with TIGIT Mouse Monoclonal Antibody (TIGIT/3106). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary antibody, secondary only negative control.
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with TIGIT Mouse Monoclonal Antibody (TIGIT/3106). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary antibody, secondary only negative control.
SDS-PAGE Analysis Purified TIGIT-Monospecific Mouse Monoclonal Antibody (TIGIT/3106). Confirmation of Integrity and Purity of Antibody.
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