Human Pepsinogen C (PGC) ELISA Kit

Catalog Number: BYT-ORB776190
Article Name: Human Pepsinogen C (PGC) ELISA Kit
Biozol Catalog Number: BYT-ORB776190
Supplier Catalog Number: orb776190
Alternative Catalog Number: BYT-ORB776190-48, BYT-ORB776190-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: PG-C ELISA Kit, Progastricsin ELISA Kit, Gastricsin ELISA Kit, Pepsinogen II ELISA Kit, Pepsinogen C ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen C(PGC).
Concentration: 60 ng/mL
Range: 0.94-60 ng/mL
Sensitivity: 0.41 ng/mL
UniProt: P20142
Samples: Serum, plasma, tissue homogenates and other biological fluids
Application Notes: Application Notes: standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PGC in the samples is then determined by comparing the OD of the samples to the standard curve