Rat N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) ELISA Kit
Biozol Catalog Number:
BYT-ORB776264
Supplier Catalog Number:
orb776264
Alternative Catalog Number:
BYT-ORB776264-48, BYT-ORB776264-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
NT-Pro-ANP ELISA Kit, N-ANP ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Atrial Natriuretic Peptide(NT-ProANP).
Concentration:
40 ng/mL
Range:
0.63-40 ng/mL
Sensitivity:
0.258 ng/mL
Samples:
Serum, plasma, tissue homogenates and other biological fluids.
Application Notes:
Application Notes: standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve
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