Rat Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit
Biozol Catalog Number:
BYT-ORB776449
Supplier Catalog Number:
orb776449
Alternative Catalog Number:
BYT-ORB776449-48, BYT-ORB776449-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
SERPINE1 ELISA Kit, PLANH1 ELISA Kit, Serpin Peptidase Inhibitor Clade E Member 1 ELISA Kit, Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1 ELISA Kit, Endothelial plasminogen activator inhibitor ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1(PAI1).
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve
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