This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Triiodothyronine(fT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Triiodothyronine(fT3).
Concentration:
100 pg/mL
Range:
1.56-100 pg/mL
Sensitivity:
0.51 pg/mL
Samples:
Serum, plasma and other biological fluids
Application Notes:
Application Notes: standard: 100 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with fT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to fT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve
orb776965
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