Human Semaphorin 7A (SEMA7A) ELISA Kit

Catalog Number: BYT-ORB776991
Article Name: Human Semaphorin 7A (SEMA7A) ELISA Kit
Biozol Catalog Number: BYT-ORB776991
Supplier Catalog Number: orb776991
Alternative Catalog Number: BYT-ORB776991-48, BYT-ORB776991-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: CD108 ELISA Kit, CDw108 ELISA Kit, H-SEMA-K1 ELISA Kit, H-Sema-L ELISA Kit, JMH ELISA Kit, SEMAK1 ELISA Kit, SEMAL ELISA Kit, Semaphorin 7A,GPI Membrane Anchor ELISA Kit, John Milton Hagen Blood Group ELISA Kit, Semaphorin-K1 ELISA Kit, Semaphorin-L ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A(SEMA7A).
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.057 ng/mL
UniProt: O75326
Samples: serum, plasma, tissue homogenates and other biological fluids
Application Notes: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SEMA7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SEMA7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SEMA7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human SEMA7A in the samples is then determined by comparing the OD of the samples to the standard curve
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