Human Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit
Biozol Catalog Number:
BYT-ORB777091
Supplier Catalog Number:
orb777091
Alternative Catalog Number:
BYT-ORB777091-48, BYT-ORB777091-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
LGIL4 ELISA Kit, LGI1-like protein 4 ELISA Kit, Leucine-rich glioma-inactivated protein 3 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat LGI Family, Member 3(LGI3).
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LGI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LGI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LGI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human LGI3 in the samples is then determined by comparing the OD of the samples to the standard curve
orb777091
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