Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit

Product Overview

• Article Name: Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit
• Biozol Catalog Number: BYT-ORB777171
• Supplier Catalog Number: orb777171
• Alternative Catalog Number: BYT-ORB777171-48, BYT-ORB777171-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: PDI-A5 ELISA Kit, PDIR ELISA Kit, Protein Disulfide Isomerase-Associated 5 ELISA Kit, Protein disulfide isomerase-related protein ELISA Kit

Product Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A5(PDIA5).

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• UniProt: Q14554
• Samples: Tissue homogenates and other biological fluids.

Application Notes

• Application Notes: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PDIA5 in the samples is then determined by comparing the OD of the samples to the standard curve