Rat Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit
Biozol Catalog Number:
BYT-ORB780045
Supplier Catalog Number:
orb780045
Alternative Catalog Number:
BYT-ORB780045-48, BYT-ORB780045-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
A-FABP ELISA Kit, AFABP ELISA Kit, AP2 ELISA Kit, Adipocyte Protein 2 ELISA Kit, Adipocyte-type fatty acid-binding protein ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 4, Adipocyte(FABP4).
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Application Notes:
Application Notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FABP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FABP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FABP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat FABP4 in the samples is then determined by comparing the OD of the samples to the standard curve
* VAT and and shipping costs not included. Errors and price changes excepted
