Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit
Biozol Catalog Number:
BYT-ORB780057
Supplier Catalog Number:
orb780057
Alternative Catalog Number:
BYT-ORB780057-48, BYT-ORB780057-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
Proto-Oncogene Wnt7a Protein ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 7A(WNT7A).
tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human WNT7A in the samples is then determined by comparing the OD of the samples to the standard curve
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