Rat Glycoprotein 2, Zymogen Granule Membrane (GP2) ELISA Kit
Biozol Catalog Number:
BYT-ORB780168
Supplier Catalog Number:
orb780168
Alternative Catalog Number:
BYT-ORB780168-48, BYT-ORB780168-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
ZAP75 ELISA Kit, Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 ELISA Kit, Pancreatic zymogen granule membrane protein GP-2 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 2, Zymogen Granule Membrane(GP2).
Serum, plasma, tissue homogenates and other biological fluids.
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat GP2 in the samples is then determined by comparing the OD of the samples to the standard curve
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