Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

Product Overview

• Article Name: Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit
• Biozol Catalog Number: BYT-ORB780180
• Supplier Catalog Number: orb780180
• Alternative Catalog Number: BYT-ORB780180-48, BYT-ORB780180-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: Gastric H,K-ATPase Alpha Subunit ELISA Kit, H(+)-K(+)-ATPase Alpha Subunit ELISA Kit, Proton Pump ELISA Kit, Potassium-transporting ATPase alpha chain 1 ELISA Kit

Product Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide(ATP4a).

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.053 ng/mL
• UniProt: Q64436
• Samples: Tissue homogenates and other biological fluids.

Application Notes

• Application Notes: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATP4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATP4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATP4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ATP4a in the samples is then determined by comparing the OD of the samples to the standard curve