Human Placenta Specific Protein 9 (PLAC9) ELISA Kit
Biozol Catalog Number:
BYT-ORB780741
Supplier Catalog Number:
orb780741
Alternative Catalog Number:
BYT-ORB780741-48, BYT-ORB780741-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
PLAC9, Placenta-specific protein 9
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Specific Protein 9(PLAC9).
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Application Notes:
Application Notes: standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLAC9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLAC9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLAC9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PLAC9 in the samples is then determined by comparing the OD of the samples to the standard curve
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