Rat Phosphorylated Neurofilament H (pNF-H) ELISA Kit
Biozol Catalog Number:
BYT-ORB780759
Supplier Catalog Number:
orb780759
Alternative Catalog Number:
BYT-ORB780759-48, BYT-ORB780759-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
NFH ELISA Kit, 200 kDa neurofilament protein ELISA Kit, Neurofilament triplet H protein ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide(NEFH).
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
5.5 pg/mL
Samples:
serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Application Notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve
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