Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit
Biozol Catalog Number:
BYT-ORB780846
Supplier Catalog Number:
orb780846
Alternative Catalog Number:
BYT-ORB780846-48, BYT-ORB780846-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
CGI-PDE ELISA Kit, Cyclic GMP-inhibited phosphodiesterase A ELISA Kit, cGMP-inhibited 3,5-cyclic phosphodiesterase A ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 3A, cGMP Inhibited(PDE3A).
Application Notes: standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDE3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PDE3A in the samples is then determined by comparing the OD of the samples to the standard curve
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