Human Neuroblastoma, Suppression of Tumorigenicity 1 (NBL1) ELISA Kit
Biozol Catalog Number:
BYT-ORB780858
Supplier Catalog Number:
orb780858
Alternative Catalog Number:
BYT-ORB780858-48, BYT-ORB780858-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
NB ELISA Kit, DAN ELISA Kit, NO3 ELISA Kit, DAND1 ELISA Kit, Differential Screening-Selected Gene Aberrant In Neuroblastoma ELISA Kit, DAN domain family member 1 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1(NBL1).
Tissue homogenates, cell lysates and other biological fluids.
Application Notes:
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NBL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NBL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NBL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NBL1 in the samples is then determined by comparing the OD of the samples to the standard curve
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