Rat Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit
Biozol Catalog Number:
BYT-ORB780867
Supplier Catalog Number:
orb780867
Alternative Catalog Number:
BYT-ORB780867-48, BYT-ORB780867-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
CPT1-L ELISA Kit, Carnitine O-palmitoyltransferase I, liver isoform ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Palmitoyltransferase 1A, Liver(CPT1A).
serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Application Notes: standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CPT1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CPT1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CPT1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat CPT1A in the samples is then determined by comparing the OD of the samples to the standard curve
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