Mouse Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) ELISA Kit
Biozol Catalog Number:
BYT-ORB780910
Supplier Catalog Number:
orb780910
Alternative Catalog Number:
BYT-ORB780910-48, BYT-ORB780910-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Mouse
Alternative Names:
THIL ELISA Kit, ACAT ELISA Kit, T2 ELISA Kit, MAT ELISA Kit, Acetoacetyl Coenzyme A Thiolase ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 1(ACAT1).
Tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ACAT1 in the samples is then determined by comparing the OD of the samples to the standard curve
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