Rat Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit
Biozol Catalog Number:
BYT-ORB780936
Supplier Catalog Number:
orb780936
Alternative Catalog Number:
BYT-ORB780936-48, BYT-ORB780936-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
ZPA ELISA Kit, Zona Pellucida Sperm-Binding Protein 2 ELISA Kit, Zona pellucida protein A ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zona Pellucida Glycoprotein 2, Sperm Receptor(ZP2).
Tissue homogenates, cell lysates and other biological fluids.
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ZP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ZP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ZP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat ZP2 in the samples is then determined by comparing the OD of the samples to the standard curve
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