Human Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) ELISA Kit

Product Overview

• Article Name: Human Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) ELISA Kit
• Biozol Catalog Number: BYT-ORB780998
• Supplier Catalog Number: orb780998
• Alternative Catalog Number: BYT-ORB780998-48, BYT-ORB780998-96
• Manufacturer: Biorbyt
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, S6K-alpha 1 ELISA Kit, 90 kDa ribosomal protein S6 kinase 1 ELISA Kit, MAP kinase-activated protein kinase 1a ELISA Kit, Ribosomal S6 kinase 1 ELISA Kit

Product Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Alpha 1(RPS6Ka1).

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.122 ng/mL
• UniProt: Q15418
• Samples: Tissue homogenates, cell lysates and other biological fluids.

Application Notes

• Application Notes: Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RPS6Ka1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RPS6Ka1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RPS6Ka1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human RPS6Ka1 in the samples is then determined by comparing the OD of the samples to the standard curve