Human Mitochondrial Open Reading Frame of the 12S rRNA-c (MOTS-c) ELISA Kit
Biozol Catalog Number:
BYT-ORB781469
Supplier Catalog Number:
orb781469
Alternative Catalog Number:
BYT-ORB781469-48, BYT-ORB781469-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
MOTS-c
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c).
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve
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