Mouse Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit
Biozol Catalog Number:
BYT-ORB781504
Supplier Catalog Number:
orb781504
Alternative Catalog Number:
BYT-ORB781504-48, BYT-ORB781504-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Mouse
Alternative Names:
Protein Wnt 3a Precursor, Protein Wnt-3a, Wnt 3a, wnt3a, WNT3A protein,
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3A(WNT3A).
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WNT3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WNT3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WNT3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse WNT3A in the samples is then determined by comparing the OD of the samples to the standard curve
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