Rat Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit

Catalog Number: BYT-ORB781521
Article Name: Rat Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit
Biozol Catalog Number: BYT-ORB781521
Supplier Catalog Number: orb781521
Alternative Catalog Number: BYT-ORB781521-48, BYT-ORB781521-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Rat
Alternative Names: P3CP ELISA Kit, C-Propeptide Of Type III Procollagen ELISA Kit, Procollagen III Carboxy Terminal Propeptide ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Procollagen III C-Terminal Propeptide(PIIICP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III C-Terminal Propeptide(PIIICP).
Concentration: 200 ng/mL
Range: 3.13-200 ng/mL
Sensitivity: 0.83 ng/mL
Samples: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes: Application Notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIIICP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve