Rat Cytochrome C Oxidase Subunit II (COX2) ELISA Kit
Biozol Catalog Number:
BYT-ORB781555
Supplier Catalog Number:
orb781555
Alternative Catalog Number:
BYT-ORB781555-48, BYT-ORB781555-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
MTCO2 ELISA Kit, MT-CO2 ELISA Kit, COII ELISA Kit, COXII ELISA Kit, Mitochondrially Encoded Cytochrome C Oxidase II ELISA Kit, Cytochrome c oxidase polypeptide II ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit II(COX2).
tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat COX2 in the samples is then determined by comparing the OD of the samples to the standard curve
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