Cattle Postacrosomal Sheath WW Domain Binding Protein (PAWP) ELISA Kit
Biozol Catalog Number:
BYT-ORB781841
Supplier Catalog Number:
orb781841
Alternative Catalog Number:
BYT-ORB781841-48, BYT-ORB781841-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Bovine
Alternative Names:
WBP2NL ELISA Kit, WBP2 N-Terminal Like ELISA Kit, WW domain-binding protein 2-like ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Postacrosomal Sheath WW Domain Binding Protein(PAWP).
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PAWP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PAWP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PAWP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle PAWP in the samples is then determined by comparing the OD of the samples to the standard curve
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