Rat JNK1/MAPK8 Associated Membrane Protein (JKAMP) ELISA Kit
Biozol Catalog Number:
BYT-ORB781872
Supplier Catalog Number:
orb781872
Alternative Catalog Number:
BYT-ORB781872-48, BYT-ORB781872-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
JAMP ELISA Kit, C14orf100 ELISA Kit, Jun N-Terminal Kinase 1-Associated Membrane Protein ELISA Kit, Medulloblastoma antigen MU-MB-50.4 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to JNK1/MAPK8 Associated Membrane Protein(JKAMP).
Concentration:
100 ng/mL
Range:
1.57-100 ng/mL
Sensitivity:
0.57 ng/mL
Samples:
tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat JKAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat JKAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat JKAMP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat JKAMP in the samples is then determined by comparing the OD of the samples to the standard curve
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