Human Anti-Heparin/Platelet Factor 4 Antibody (Anti-HPF4) ELISA Kit
Biozol Catalog Number:
BYT-ORB781874
Supplier Catalog Number:
orb781874
Alternative Catalog Number:
BYT-ORB781874-48, BYT-ORB781874-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
HPF4
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Heparin/Platelet Factor 4 Antibody(Anti-HPF4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Heparin/Platelet Factor 4 Antibody(Anti-HPF4).
Concentration:
100 ng/mL
Range:
1.57-100 ng/mL
Sensitivity:
0.58 ng/mL
Samples:
Serum, plasma, other biological fluids
Application Notes:
Application Notes: standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve
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