ZSIG9 ELISA Kit, Cnpy2 ELISA Kit, MSAP ELISA Kit, TMEM4 ELISA Kit, Transmembrane Protein 4 ELISA Kit, MIR-interacting saposin-like protein ELISA Kit, Putative secreted protein Zsig9 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Canopy 2 Homolog(CNPY2).
Tissue homogenates, cell lysates and other biological fluids.
Application Notes:
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CNPY2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CNPY2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CNPY2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse CNPY2 in the samples is then determined by comparing the OD of the samples to the standard curve
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