Mouse Wingless Type MMTV Integration Site Family, Member 16 (WNT16) ELISA Kit
Biozol Catalog Number:
BYT-ORB781965
Supplier Catalog Number:
orb781965
Alternative Catalog Number:
BYT-ORB781965-48, BYT-ORB781965-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Mouse
Alternative Names:
Protein Wnt 16 precursor, Protein Wnt-16, WNT16,
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 16(WNT16).
tissue homogenates, cell lysates and other biological fluids
Application Notes:
Application Notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WNT16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WNT16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WNT16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse WNT16 in the samples is then determined by comparing the OD of the samples to the standard curve
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