Rat Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) ELISA Kit
Biozol Catalog Number:
BYT-ORB782012
Supplier Catalog Number:
orb782012
Alternative Catalog Number:
BYT-ORB782012-48, BYT-ORB782012-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
ZA2G ELISA Kit, ZAG ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding(aZGP1).
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat aZGP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat aZGP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat aZGP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat aZGP1 in the samples is then determined by comparing the OD of the samples to the standard curve
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