Rat Mitochondrial Open Reading Frame of the 12S rRNA-c (MOTS-c) ELISA Kit
Biozol Catalog Number:
BYT-ORB782062
Supplier Catalog Number:
orb782062
Alternative Catalog Number:
BYT-ORB782062-48, BYT-ORB782062-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
MOTS-c
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c).
Concentration:
200 ng/mL
Range:
3.13-200 ng/mL
Sensitivity:
0.97 ng/mL
Samples:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve
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