Rat Mothers Against Decapentaplegic Homolog 5 (Smad5) ELISA Kit
Biozol Catalog Number:
BYT-ORB782430
Supplier Catalog Number:
orb782430
Alternative Catalog Number:
BYT-ORB782430-48, BYT-ORB782430-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rat
Alternative Names:
MADH5 ELISA Kit, Dwfc ELISA Kit, JV5-1 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 5(Smad5).
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Smad5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Smad5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Smad5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat Smad5 in the samples is then determined by comparing the OD of the samples to the standard curve
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