Mouse Major Histocompatibility Complex Class II (MHCII) ELISA Kit
Biozol Catalog Number:
BYT-ORB782477
Supplier Catalog Number:
orb782477
Alternative Catalog Number:
BYT-ORB782477-48, BYT-ORB782477-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Mouse
Alternative Names:
Major histocompatibility complex class II ELISA Kit, MHCII ELISA Kit, HLA-DR ELISA Kit, MHC cell surface glycoprotein ELISA Kit, MHC class II antigen DRA ELISA Kit, MHC II ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II(MHCII).
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
6.2 pg/mL
Samples:
Serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Application Notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MHCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MHCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MHCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse MHCII in the samples is then determined by comparing the OD of the samples to the standard curve
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