This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Humanin-Like Protein 6 (MTRNR2L6) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Humanin-Like Protein 6 (MTRNR2L6).
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTRNR2L6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTRNR2L6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MTRNR2L6 in the samples is then determined by comparing the OD of the samples to the standard curve
orb782561
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