Human Vesicle Associated Membrane Protein 1 (VAMP1) ELISA Kit

Catalog Number: BYT-ORB782601
Article Name: Human Vesicle Associated Membrane Protein 1 (VAMP1) ELISA Kit
Biozol Catalog Number: BYT-ORB782601
Supplier Catalog Number: orb782601
Alternative Catalog Number: BYT-ORB782601-48, BYT-ORB782601-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: SYB1 ELISA Kit, VAMP-1 ELISA Kit, ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicle Associated Membrane Protein 1(VAMP1).
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 5.8 pg/mL
UniProt: P23763
Samples: Tissue homogenates, cell lysates and other biological fluids.
Application Notes: Application Notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VAMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VAMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VAMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human VAMP1 in the samples is then determined by comparing the OD of the samples to the standard curve
orb782601