Sheep Surfactant Associated Protein B (SPB) ELISA Kit
Biozol Catalog Number:
BYT-ORB782669
Supplier Catalog Number:
orb782669
Alternative Catalog Number:
BYT-ORB782669-48, BYT-ORB782669-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Sheep
Alternative Names:
SFTPB ELISA Kit, PSPB ELISA Kit, SFTB3 ELISA Kit, SFTP3 ELISA Kit, SP-B ELISA Kit, Pulmonary Surfactant Protein B ELISA Kit, 18 kDa pulmonary-surfactant protein ELISA Kit, 6 kDa protein ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein B(SPB).
Concentration:
100 ng/mL
Range:
1.57-100 ng/mL
Sensitivity:
0.56 ng/mL
Samples:
serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Application Notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep SPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sheep SPB in the samples is then determined by comparing the OD of the samples to the standard curve
orb782669
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