Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB783427
• Supplier Catalog Number: orb783427
• Alternative Catalog Number: BYT-ORB783427-100,BYT-ORB783427-200,BYT-ORB783427-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad2 around the phosphorylation site of Ser467
• Conjugation: Unconjugated
• Alternative Names: Smad2(phospho S467), p-Smad2(phospho S467), hMAD 2, hMAD-2, hSMAD2, JV18 1, JV18, JV18-1, JV181, MAD, MAD homolog 2, MAD Related Protein 2, Mad-related protein 2, MADH2, MADR2, MGC22139, MGC34440, Mother against DPP homolog 2, Mothers Against Decapentaplegic Homolog 2, mothers against DPP homolog 2, OTTHUMP00000163489, Sma and Mad related protein 2, SMAD 2, SMAD, SMAD family member 2, SMAD, mothers against DPP homolog 2, SMAD2, SMAD2_HUMAN.

Product Description

Phospho-Smad2 (Ser467) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 58 kDa
• UniProt: Q15796
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SMAD2

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-Smad2 (Ser467)) polyclonal Antibody, Unconjugated (orb783427) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Smad2 (Ser467)) Polyclonal Antibody, Unconjugated (orb783427) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of rat placenta using phospho-Smad2 Ser467 Polyclonal Antibody at 1:200 dilution.