GPR54/KISS1R Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GPR54/KISS1R Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB865425
• Supplier Catalog Number: orb865425
• Alternative Catalog Number: BYT-ORB865425-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: E.coli-derived human GPR54/KISS1R recombinant protein (Position: N60-A373).
• Conjugation: Unconjugated
• Alternative Names: KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, hOT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, KISS1R, AXOR12, GPR54

Product Description

Anti-GPR54/KISS1R Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Properties

• Clonality: Polyclonal
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molecular Weight: 80 kDa
• UniProt: Q969F8
• Form: Lyophilized

Application Notes

• Application Notes: Application Notes: Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml

Images

IF analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-GPR54/KISS1R Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human hyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then in