Phospho-MLK3 (Thr277 + Ser281) Rabbit Polyclonal Antibody (FITC)

Product Overview

• Article Name: Phospho-MLK3 (Thr277 + Ser281) Rabbit Polyclonal Antibody (FITC)
• Biozol Catalog Number: BYT-ORB9272
• Supplier Catalog Number: orb9272
• Alternative Catalog Number: BYT-ORB9272-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human MLK3 around the phosphorylation site of Thr277/Ser281 K(p-T)TQM(p-S)AA
• Conjugation: FITC
• Alternative Names: MLK3(phospho T277+S281), MEKK11, MGC17114, Mitogen-activated protein kinase kinase kinase 11, Mixed lineage kinase 3, Mixed lineage protein kinase 3, Mlk-3, Mlk3, Protein tyrosine kinase PTK1, PTK1, RHOE, SH3 domain containing proline rich kinase, SPRK, Src-homology 3 domain-containing proline-rich kinase, 2610017K16Rik, EC 2.7.11.25, M3K11_HUMAN.

Product Description

Phospho-MLK3 (Thr277 + Ser281) Rabbit Polyclonal Antibody (FITC) is an FITC conjugated antibody targeting MAP3K11. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse, Rat samples.

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Target: MAP3K11

Application Notes

• Application Dilute: Flow-Cyt=1ug/Test, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Blank control: A431. Primary Antibody (green line): Rabbit Anti-MLK3 antibody (orb9272), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-MLK3 (Thr277 + Ser281) antibody (orb9272), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-Phospho-MLK3 (Thr277 + Ser281) antibody (orb9272), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

FC analysis of NIH/3T3 cell lysate using MLK3 (phospho-Thr277/Ser281) antibody (FITC)

FC analysis of A431 cell lysate using MLK3 (phospho-Thr277/Ser281) antibody (FITC)

FC analysis of A431 cell lysate using MLK3 (phospho-Thr277/Ser281) antibody (FITC)