DAG (Diacylglycerol) Assay Kit

Product Overview

• Article Name: DAG (Diacylglycerol) Assay Kit
• Biozol Catalog Number: CBL-MET-5028
• Supplier Catalog Number: MET-5028
• Alternative Catalog Number: CBL-MET-5028
• Manufacturer: Cell Biolabs
• Category: Kits/Assays

Product Description

Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kits Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.