NAD+/NADH Assay Kit (Fluorometric)

Catalog Number: CBL-MET-5030
Article Name: NAD+/NADH Assay Kit (Fluorometric)
Biozol Catalog Number: CBL-MET-5030
Supplier Catalog Number: MET-5030
Alternative Catalog Number: CBL-MET-5030
Manufacturer: Cell Biolabs
Category: Kits/Assays
Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a fluorometric probe and is detected with a fluorescence plate reader at 530-570nm excitation and 590-600nm emission. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.