OxiSelect(TM) Cellular UV-Induced DNA Damage Staining Kit (CPD)

Catalog Number: CBL-STA-327
Article Name: OxiSelect(TM) Cellular UV-Induced DNA Damage Staining Kit (CPD)
Biozol Catalog Number: CBL-STA-327
Supplier Catalog Number: STA-327
Alternative Catalog Number: CBL-STA-327
Manufacturer: Cell Biolabs
Category: Kits/Assays
Application: Fluorescence Microscopy
Our OxiSelect(TM) Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.