OxiSelect(TM) Intracellular ROS Assay Kit (Green Fluorescence)
Catalog Number:
CBL-STA-342-5
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| Article Name: | OxiSelect(TM) Intracellular ROS Assay Kit (Green Fluorescence) |
| Biozol Catalog Number: | CBL-STA-342-5 |
| Supplier Catalog Number: | STA-342-5 |
| Alternative Catalog Number: | CBL-STA-342-5 |
| Manufacturer: | Cell Biolabs |
| Category: | Kits/Assays |
| Application: | Fluorometric |
| The OxiSelect(TM) ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader. |
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