Y1HGold-pAbAi Yeast 1 Hybrid Interaction Validation Kit Preis auf Anfrage
Biozol Catalog Number:
CRB-HN-1224-TT5-1
Supplier Catalog Number:
HN-1224-TT5
Alternative Catalog Number:
CRB-HN-1224-TT5-1
Manufacturer:
Creative Biolabs
Category:
Kits/Assays
The strian used for Y1HGold-pAbAi Y1H system is YIHGold MATa type, wihch can be directly transformed into plasmids for screening experiments. Screening markers are: ura3, leu2 and reporter is AbAr. The YIHGold-GAL4-AbA yeast one-hybrid system requires the use of two plasmids, pAbAi and pGADT7. The selection marker of plasmid pAbAi is URA, which is used to express pBait-AbAi construct (1 to 3 bait DNA sequences are repeated in series and cloned into pAbAi), the selection marker of plasmid pGADT7 is LEU, which is used to express the fusion protein of AD (Amino acids 768-881 at the C-terminus of GAL4) and target protein (Prev).
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