Native Bovine Trypsin: 2,500 USP U/mg, CAS [[07/07/9002]]
Biozol Catalog Number:
CRM-NATE-0721
Supplier Catalog Number:
NATE-0721
Alternative Catalog Number:
CRM-NATE-0721-1
Manufacturer:
Creative BioMart
Category:
Biochemikalien
Concentration:
2,500 USP U/mg
CAS Number:
[07/07/9002]
Application Notes:
For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps. Trypsin can be used to release adherent cells from tissue culture plates for passaging. Trypsin has been used in a study to assess the effects of macromolecular crowding on the structural stability of human alpha-lactalbumin. Trypsin has also been used in a study to investigate BN-PAGE analysis of Trichoderma harzianum secretome.
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